Partial purification of a factor from human synovium that possesses interleukin 1, chondrocyte stimulating and catabolin‐like activities

FEBS Letters

Elford, Peter R.¹  Russell, R.Graham G.¹  Meats, Judith E.¹  Sharrard, R.Michael¹ 

[1] Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, Beach Hill Road, Sheffield S10 2RX, England

关键词: Cartilage;    Chondrocyte;    Interleukin 1;    Prostaglandin E;    Rheumatoid arthritis;    Synovium;    PGE 2;    prostaglandin E2;    IL-1;    interleukin 1;    PA;    plasminogen activator;    FCS;    fetal calf serum;    LAF;    lymphocyte activating factor;   

DOI  :  10.1016/0014-5793(85)80528-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human synovial explants in culture release material that stimulates the production of prostaglandin E₂ (PGE₂) and several extracellular enzymes by human chondrocytes. Fractionation of conditioned medium by gel filtration revealed a protein of approx. 15 kDa, which in addition to stimulating production of PGE₂ and plasminogen activator by human articular chondrocytes, possessed interleukin 1 activity and induced cartilage degradation. Further purification using iso-electric focussing again showed co-elution of these activities with a major pI of 6.9 and a minor pI of 5.1–5.3. This study indicated that human synovium releases a facor that is closely related to or identical with interleukin 1 and suggests that this protein may participate in cellular interactions that occur within the rheumatoid joint.

【 授权许可】

Unknown