In vitro reconstitution of messenger ribonucleoprotein particles from globin messenger RNA and cytosol proteins

FEBS Letters

Setyono, B.¹  Gaedigk, R.¹  Köhler, K.¹  Oehler, S.¹ 

[1] Biologisches Institut der Universität Stuttgart, Abteilung Zoologie, Ulmerstrasse 227, D-7000 Stuttgart 60, FRG

关键词: Messenger ribonucleoprotein;    Poly(A)-protein;    Ultraviolet-light cross-linking;    RNA-binding protein;    mRNP;    messenger RNA-ribonucleoprotein complex;    Mes;    N-morpholinoethanesulfonic acid;    DMSO;    dimethyl sulfoxide;    PAGE;    polyacrylamide gel electrophoresis;    BP350;    cytosol RNA-binding proteins eluted from the RNA-Sepharose column with buffered 350 mM KC1;    BP1000;    cytosol RNA-binding proteins eluted from the RNA-Sepharose column with buffered 1000 mM KC1;   

DOI  :  10.1016/0014-5793(85)80518-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs₂SO₄ and the composition of proteins which can be crosslinked to the mRNA are concerned. Proteins thus identified bind specifically to mRNA and not to ribosomal RNA or any synthetic oligonucleotides, with one exception: a 78-kDa protein could be cross-linked to poly(A).

【 授权许可】

Unknown