Identification of the proteins in direct contact with duck globin mRNA

FEBS Letters

Lockard, Raymond E.¹ 

[1] Department of Biochemistry, Cornell University Medical College, 1300 York Ave, New York, NY 10021, USA

关键词: Reticulocyte;    Messenger ribonucleoprotein;    Ultraviolet cross-linking;    Prosome;    (Duck);   

DOI  :  10.1016/0014-5793(87)80262-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteins in direct contact with translationally active and repressed duck globin mRNA were determined by irradiating blood or lysates with ultraviolet light. Cross-linked proteins from polyribosomes and free mRNP particles were ¹⁴C-labeled by reductive methylation and identified on SDS-polyacrylamide gels upon autoradiography. Results indicate that ten cross-linked proteins are common to both polysomal and free mRNP, however, a 44 kDa protein appears to be specific for repressed mRNP particles. Furthermore, the notable lack of cross-linked proteins in the 20–30 kDa range in free mRNP supports the view that the characteristic low molecular mass ‘prosomal’ proteins, previously found associated with translationally repressed duck globin free mRNP [(1984) EMBO J. 3, 29–34], do not interact directly with the mRNA molecule.

【 授权许可】

Unknown