Picosecond time‐resolved fluorescence spectra of photosystem I and II in Chlorella pyrenoidosa

FEBS Letters

Fujita, Y.²  Yamazaki, I.¹  Tamai, N.¹  Yamazaki, T.¹  Mimuro, M.² 

[1] Institute for Molecular Science, Myodaiji, Okazaki 444, Japan;National Institute for Basic Biology, Myodaiji, Okazaki 444, Japan

关键词: Chlorella pyrenoidosa;    Photosystem I;    Time-resolved fluorescence spectrum;    Chlorophyll-protein complex;    Energy transfer Photosynthesis;    PS;    photosystem;    RC;    reaction center;    Chl a;    chlorophyll a;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;   

DOI  :  10.1016/0014-5793(85)80192-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Picosecond time-resolved fluorescence spectra emitted from intact cells of the green alga Chlorella pyrenoidosa have been measured by means of a new detection technique using a microchannel-plate photomultiplier. A fluorescence band (F700) was observed at 690–730 nm in the initial time region (0–180 ps), in addition to the well-known spectrum (F685) of photosystem II (PS II)-chlorophyll a (Chla) with a peak at 685 nm. F700 decays rapidly with lifetime of 104 ps, while F685 decays much more slowly in bi-exponential form with lifetimes of 0.64 and 1.7 ns. Appearance of F700 is independent of closure of the reaction center II (RC II). F700 is thus assigned to the fluorescence from PS I-Chl a, whose decay is governed by a fast energy transfer process from the antenna Chl aof PS I to P700 of RC I.

【 授权许可】

Unknown