Presence and significance of minor antenna components in the energy transfer sequence of the green photosynthetic bacterium Chloroflexus aurantiacus

FEBS Letters

Yamazaki, Iwao⁴  Nozawa, Tsunenori³  Nishimura, Yoshinobu⁴  Mimuro, Mamoru¹  Tamai, Naoto² 

[1] National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444, Japan;Microphotoconversion Project, ERATO, Research Development Corporation of Japan, Shimogamo, Sakyo-ku, Kyoto 606 Japan;Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University, Aoba, Sendai, Miyagi 980, Japan;Department of Chemical Process Engineering, Faculty of Engineering, Hokkaido University, Sapporo 060, Japan

关键词: Bacteriochlorophyll;    Energy transfer;    Green photosynthetic bacteria;    Photosynthesis;    Pigment system;    Time-resolved fluorescence spectrum;    Chloroflexus aurantiacus;    Bchl;    bacteriochlorophyll;    CD;    circular dichroism;    F765;    a representative expression of fluorescence component located at 765 nm;    LD;    linear dichroism;    LH;    light harvesting;    RC;    reaction center;   

DOI  :  10.1016/0014-5793(94)80130-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antenna components in the energy transfer processes of a green photosynthetic bacterium Chloroflexus aurantiacus were spectrally investigated by time-resolved fluorescence spectroscopy at −196°C on intact cells. Besides major antenna components so far reported, three minor components were resolved; those were Bchl c located at 785 nm, the baseplate Bchl a at 819 nm and Bchl a in the B808-866 complex at 910 nm. The last component was assigned to a longer wavelength antenna closely associated with a reaction center. An additional Bchl c fluorescence component was kinetically suggested to be present, which can be an energy donor to a major Bchl c. Presence of these minor components was signified in terms of (1) increase in the spectral overlap integral and (2) adjustment of the direction of dipole moments in the energy transfer sequence of intact cells.

【 授权许可】

Unknown