Biological activities of human recombinant interferon α/β targeted by anti‐Epstein‐Barr virus monoclonal antibodies

FEBS Letters

Alkan, S.S.¹  Braun, D.G.¹  Miescher-Granger, S.¹  Hochkeppel, H.K.¹ 

[1] Pharmaceutical Research Laboratories, Ciba-Geigy Limited, CH-4002 Basel, Switzerland

关键词: Recombinant interferon;    Monoclonal antibody;    Targeting;    Growth inhibition;    Viral protection;   

DOI  :  10.1016/0014-5793(85)80184-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The requirement of high doses of interferon (IFN) during therapy severely restrict its application. Thus a model using an Epstein-Barr virus (EBV) membrane antigen (MA) specific monoclonal antibody (MAb) was developed to assess the feasibility of coupling minimal amounts of IFN to a MAb and specifically delivering the IFN to the target cells. Coupled IFN was first shown to retain fully both its anti-viral and antiproliferative properties when tested on human tumor cell lines QIMR-WIL (EBV-MA⁺) and the U-266 (EBV-MA⁻¹). A series of in vitro pulsing experiments demonstrated the specific targeting of both the anti-viral and anti-proliferative properties of IFN to the EBV-MA⁺ QIMR-WIL cells and not EBV-MA⁻¹ cell lines.

【 授权许可】

Unknown