The antilipolytic effect of insulin does not require adenylate cyclase or phosphodiesterase action

FEBS Letters

Gabbay, Robert A.¹  Lardy, Henry A.¹ 

[1] Institute for Enzyme Research and Department of Biochemistry, University of Wisconsin, Madison, WI 53705, USA

关键词: Lipolysis;    Rat adipocyte;    Insulin;    Phosphodiesterase;    Adenylate cyclase;    Cyclic AMP;    Ro 20-1724;    d-4-(3-butoxy-4-methoxybenzyl)-2-imidizolidione;   

DOI  :  10.1016/0014-5793(85)80179-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin antagonized the lipolytic actions of epinephrine in rat epididymal adipocytes when the phosphodiesterase inhibitor, Ro 20-1724, was present. Adipocytes were depleted of functional cAMP by inhibiting adenylate cyclase with N ⁶-phenylisopropyladenosine in the presence of adenosine deaminase such that Ro 20-1724 no longer stimulated lipolysis. The cAMP analogs 8-thioisopropyl-cAMP or 8-thiomethyl-cAMP, which are resistant to phosphodiesterase hydrolysis, were subsequently added to bypass adenylate cyclase and phosphodiesterase action. Under these conditions, insulin antagonized the lipolytic effects of these analogs, even in the presence of Ro 20-1724.

【 授权许可】

Unknown