Glycosidic bond rearrangements in isomeric xylobioses by yeast xylan‐degrading enzymes

FEBS Letters

Biely, Peter¹  Petráková, Eva¹ 

[1] Institute of Chemistry, Centre for Chemical Research, Slovak Academy of Sciences, CS-842 38 Bratislava, Czechoslovakia

关键词: Cryptococcus albidus;    β-Xylanase;    β-Xylosidase;    β-Xylobiose;    Transglycosylation;    Induction;    Xylβl-2Xyl;    2-O-β-D-xylopyranosyl-D-xylopyranose;    Xylβl-3Xyl;    3-O-β-D-xylopyranosyl-D-xylopyranose;    Xylβl-4Xyl;    4-O-β-D-xylopyranosyl-D-xylopyranose;    Xylβl-4Xylβl-4Xyl;    xylotriose;   

DOI  :  10.1016/0014-5793(84)80626-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cells of Cryptococcus albidus induced for xylan-degrading enzymes are capable of transforming 1,2-β-xylobiose and 1,3-β-xylobiose into 1,4-β-xylobiose, the natural inducer. The conversion involves transglyco-sylation and hydrolysis catalyzed by β-xylosidase and β-xylanase. A probable intermediate of the conversion of 1,2-β-xylobiose was isolated and identified as a trisaccharide, 4-O-β-xylopyranosyl-2-O-β-xylopyranosyl-D-xylopyranose. The trisaccharide is cleaved by purified endo-1,4-β-xylanase of C. albidus mainly at the 1,2-β-linkage yielding xylose and 1,4-β-xylobiose.

【 授权许可】

Unknown