Phorbol esters stimulate spermidine/spermine N 1‐acetyltransferase activity in mitogen‐stimulated bovine lymphocytes

FEBS Letters

Matsui-Yuasa, Isao¹  Otani, Shuzo¹  Shu, Zhao Wu¹  Morisawa, Seiji¹ 

[1] Department of Biochemistry, Osaka City University Medical School, Asahimachi, Abenoku, Osaka 545, Japan

关键词: Phorbol ester;    Spermidine/spermine;    N1-acetyltransferase;    Phytohemagglutinin;    Bovine lymphocyte;   

DOI  :  10.1016/0014-5793(84)80620-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phorbol 12-myristate-13-acetate (PMA) is shown to induce spermidine/spermine N ¹-acetyltransferase, a rate-limiting enzyme of polyamine biodegradation, in bovine lymphocytes. When PMA and phytohemagglutinin (PHA) were added simultaneously, the enzyme activity was stimulated synergistically. The ability of phorbol esters to stimulate the enzyme activity was consistent with their tumor-promoting ability. Phorbol, which is not a tumor promotor, was incapable of stimulating the enzyme activity. Phorbol diacetate weakly stimulated the activity of the acetylase. Phorbol dibutyrate had a similar stimulatory effect to PMA. These results suggest that the spermidine/spermine N ¹-acetyltransferase may play an important role in changes in polyamine levels in phorbol ester-treated cells and that the increase in the enzyme activity may have some relationship to the control of cell growth and differentiation by phorbol esters.

【 授权许可】

Unknown