Malonyl CoA inhibition of carnitine palmityltransferase in rat heart mitochondria

FEBS Letters

Shug, Austin L.¹  Paulson, Dennis J.¹  Ward, Karen M.¹ 

[1] Metabolic Research Laboratory, William S. Middleton Memorial Veterans Hospital, 2500 Overlook Terrace, Madison, WI 53705, USA

关键词: Malonyl CoA;    Carnitine;    Palmityltransferase;    Mitochondria;    Fasting heart;   

DOI  :  10.1016/0014-5793(84)81201-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects ofmalonyl CoA on carnitine palmityltransferase I (CPT-I), fatty acid-supported state 3 respiration, and carnitine reversal of palmityl CoA inhibition of state 3 respiration and of the adenine nucleotide translocator, were studied in isolated rat heart mitochondria. Malonyl CoA was a potent competitive inhibitor of CPT-I with an I ₅₀ of 0.8 μM. Fasting did not affect CPT-I activity or the I ₅₀ value of malonyl CoA. Malonyl CoA inhibited fatty acid-supported respiration and prevented carnitine from reversing the inhibition of the adenine nucleotide translocator by palmityl CoA. These findings suggest that malonyl CoA may affect fatty acid oxidation in the heart.

【 授权许可】

Unknown