A free‐radical intermediate in the reduction of plant Cu‐amine oxidases

FEBS Letters

Rinaldi, Augusto²  Rotilio, Giuseppe¹  Finazzi-Agro, Alessandro¹  Floris, Giovanni² 

[1] Institute of Biological Chemistry, University of Rome and CNR Center for Molecular Biology, P.Ie A. Moro 5, 00185 Rome, Italy;Institute of Biological Chemistry, University of Cagliari, Via della Pineta 77, 70100 Cagliari, Italy

关键词: Amine oxidase;    Free radical;    ESR;    Enzyme mechanism;    Copper protein;   

DOI  :  10.1016/0014-5793(84)81200-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The reaction with substrate of plant amine oxidases from either Euphorbia latex or lentil seedlings in the presence of cyanide led to the appearance of a free-radical type of ESR spectrum with well-resolved hyperfine structure, which is very likely due to coupling with various protons over an aromatic ring. It was established that the free-radical spectrum was not derived from the substrate, since either aromatic or aliphatic substrates gave rise to identical spectra. The presence of copper is essential to the appearance of the freeradical ESR signal.

【 授权许可】

Unknown