Evidence for a two‐step process in prostaglandin secretion

FEBS Letters

Hullin, Françoise¹  Chap, Hugues¹  Thomas, Jeannie Marie-France¹  Douste-Blazy, Louis¹ 

[1] INSERM Unité 101, Biochimie des Lipides, Hôpital Purpan, 31059 Toulouse, France

关键词: Endothelial cell;    Prostaglandin;    Prostacyclin;    Arachidonic acid;    Secretion;    Thrombin;    EC;    endothelial cells;    PG;    prostaglandin;    AA;    arachidonic acid;    ASA;    acetylsalicylic acid (aspirin);    THR;    thrombin;   

DOI  :  10.1016/0014-5793(84)80941-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cultured endothelial cells (EC) from human umbilical vein were incubated with [U-¹⁴C]arachidonic acid (AA) followed by a challenge with thrombin (2 ) or calcium ionophore A23187 (5 μM) for 0.5–10 min at 37°C. In both cases, AA was rapidly liberated from phospholipids and converted into prostaglandin I₂ (PGI₂), as determined by the radioactivity of the stable derivative 6-keto-PGF_1α. Maximal liberation of AA and synthesis of PGI₂ were-achieved within 2 min, but the two compounds first accumulated in EC prior to their release into supernatants. This finding, which was never reported before, raises the question of the mechanism of AA and PG release through the cell membranes and offers a convenient model to investigate this still obscure process.

【 授权许可】

Unknown