A gene fusion that localises the penicillin‐binding domain of penicillin‐binding protein 3 of Escherichia coli

FEBS Letters

Spratt, Brian G.¹  Hedge, Philip J.¹ 

[1] Microbial Genetics Group, School of Biological Sciences, University of Sussex, Palmer, Brighton BN1 9QG, England

关键词: Escherichia coli;    Penicillin-binding protein;    Gene fusion;    β-Lactam antibiotic;    Peptidoglycan;    Transglycosylase-transpeptidase;   

DOI  :  10.1016/0014-5793(84)80936-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene fusion that links the COOH-terminal 349 amino acids of penicillin-binding protein 3 (60 kDa) of E.coli to the NH₂-terminus of β-galactosidase has been constructed. The fusion protein (38.5 kDa) retains the ability to bind benzylpenicillin with high affinity, establishing that the penicillin-binding domain (and presumably the penicillin-sensitive transpeptidase activity) of this high molecular mass penicillin-binding protein is located on a COOH-terminal functional domain.

【 授权许可】

Unknown