Solubilization and reconstitution of the Na+‐motive NADH oxidase activity from the marine bacterium Vibrio alginolyticus

FEBS Letters

Tokuda, Hajime¹ 

[1] Research Institute for Chemobiodynamics, Chiba University, 1-8-1 Inohana, Chiba 280, Japan

关键词: NADH oxidase;    Sodium pump;    Na+ electrochemical potential;    Octylglucoside;    Reconstitution Marine bacterium;    Δψ;    membrane potential;    CCCP;    carbonylcyanide m-chlorophenylhydrazone;    HQNO;    2-heptyl-4-hydroxyquinoline-N-oxide;    MET;    2-mercaptoethanol;    octylglucoside;    octyl-β-D-glucopyranoside;    Q-1;    ubiquinone-1;   

DOI  :  10.1016/0014-5793(84)80925-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Na⁺-motive NADH oxidase activity from Vibrio alginolyticus was extracted with octylglucoside and reconstituted into liposomes by dilution. On the addition of NADH, the reconstituted proteoliposomes generated Δψ (inside positive) and ΔpH (inside alkaline) in the presence of a proton conductor CCCP, and accumulated Na⁺ in the presence of valinomycin. These results indicate that the NADH oxidase activity, reconstituted in opposite orientation, leads to the generation of an eletrochemical potential of Na⁺ by the influx of Na⁺.

【 授权许可】

Unknown