Conjugation‐dependent recovery of the Na+ pump in a mutant of Vibrio alginolyticus lacking three subunits of the Na+ pump

FEBS Letters

Yamamoto, Tomoko¹  Unemoto, Tsutomu²  Asano, Makoto²  Tokuda, Hajime²  Udagawa, Toshiaki² 

[1] School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 280, Japan;Research Institute for Chemobiodynamics Chiba University, 1-8-1 Inohana, Chiba 280, Japan

关键词: Na+ pump;    NADH oxidase;    Conjugation;    Plasmid;    (Marine bacterium);    CCCP;    carbonylcyanide m-chlorophenylhydrazone;    HQNO;    2-heptyl-4-hydroxyquinoline-N-oxide;    octylthioglucoside;    n-octyl-β-D-thioglucopyranoside;    Nal;    nalidixic acid;    Rm;    rifamycin;   

DOI  :  10.1016/0014-5793(87)80173-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Na⁺ pump-deficient mutant, Napl, of Vibrio alginolyticus was found to lack three subunits of Na⁺-dependent NADH:quinone oxidoreductase complex. Although a spontaneous Na⁺ pump positive revertant did not appear from Napl, transconjugants that recovered both the Na⁺ pump activity and the subunits were isolated from Napl conjugated with the wild type. Moreover, the wild type was found to contain two different sizes of plasmids. These results suggest the possibility that the Na⁺ pump is encoded by a plasmid.

【 授权许可】

Unknown