Chloroplastic glutamine synthetase from tobacco leaves: a glycosylated protein

FEBS Letters

Nato, Aimé¹  Gadal, Pierre¹  Nato, Farida¹  Hirel, Bartrand² 

[1] INSERM U. 180, Faculté de Médecine, 75006 ParisFrance;Physiologie Végétale Métabolique. ERA CNRS 799, Bât. 430, Université de Paris-Sud, 91405 Orsay CédexFrance

关键词: Glutamine synthetase;    Isoform;    Amino acid;    Carbohydrate;    Glycosylation;   

DOI  :  10.1016/0014-5793(84)80785-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amino acid and carbohydrate content of chloroplastic glutamine synthetase from tobacco leaves has been analysed. The enzyme subunit contanins 5% carbohydrate, mainly represented by glucosamine, galactosamine, glucose, galactose and mannose residues. The enzyme subunit displayed a single band of molecular mass 44000 Da after sodium dodecyl sulphate (SDS) electrophoresis. However, when isoelectrofocussing electrophoresis was performed, four subunits were evident differing by their charge. Furthermore, the four different subunits stained positively when tested with periodic acid Shiff reagent, showing that sugars and amino sugars were present within all the subunits.

【 授权许可】

Unknown