Kinetic analysis of glutamine synthetases from various plants

FEBS Letters

Acaster, Michael A.¹  Weitzman, P.D.J.¹ 

[1] Department of Biochemistry, University of Bath, Bath BA2 7 A Y, England

关键词: Glutamine synthetase;    Phosphinothricin;    δ-Hydroxylysine;   

DOI  :  10.1016/0014-5793(85)81031-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glutamine synthetase (GS) was partially purified from the leaves of 7 plant species and the relative contributions of two isoforms, GS1 and GS2, were determined. Only 3 species, Zea mays, Hordeum vulgare and Triticwn aesticwn, contained detectable levels of GS1. K_m values for glutamate and ATP of the various GS isolates were all within a narrow range. Both GS isoforms may be inhibited by phosphinothricin, competitively with glutamate. δ-Hydroxylysine also inhibited both isoforms but acted uncompetitively. The kinetic parameters of GS isolated from all 7 species showed a remarkable similarity.

【 授权许可】

Unknown