Increase in temperature induces the Z to B transition of poly[d(G‐C)] in water‐ethanol solution

FEBS Letters

Ivanov, Valery I.¹  Karapetyan, Armen T.²  Minyat, Elvira E.² 

[1] Institute of Molecular Biology, USSR Academy of Sciences, 117984 Moscow B-334, USSR;Kirovakan Teachers Training Institute, Kirovakan, Armenian SSR USSR Academy of Sciences, 117984 Moscow B-334, USSR

关键词: Poly[d(G-C)];    Ethidium bomide;    Z form;    Circular dichroism;    B-Z trantion enthalpy;   

DOI  :  10.1016/0014-5793(84)81055-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An increase in temperature from 20 to 50° C results in the complete transition from the Z to B form of poly(d(G-C)], dissolved in a 55% ethanol-water solution. The transition is fully reversible and displays a slow kinetics. The transition profiles for the free polynucleotide and for that in the presence of ethidium bromide, which is known to stabilize the B form, are obtained by circular dichroism. Based on these data the enthalpy value for the B-Z transition in our conditions is estimated to be ΔH _BZ = −0.7 .

【 授权许可】

Unknown