Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites

FEBS Letters

Lockyer, Michael J.¹  Odink, Karel G.¹  Holder, Anthony A.¹  Nicholls, Stephen C.¹  Freeman, Robert R.¹  Hillman, Yvonne¹ 

[1] Department of Molecular Biology, Wellcome Research Laboratories, Langley Court, Beckenham BR3 3BS, England

关键词: Human malaria;    Surface antigen;    cDNA;    Fusion polypeptide;   

DOI  :  10.1016/0014-5793(84)81027-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA library of P. falciparum was constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195-kDa protein which is the precursor to 3 major surface antigens of P. falciparum merozoites.

【 授权许可】

Unknown