Lectins facilitate calcium‐induced fusion of phospholipid vesicles containing glycosphingolipids

FEBS Letters

Papahadjopoulos, Demetrios¹  Hoekstra, Dick²  Hong, Keeking¹  Düzgünes, Nejat¹ 

[1] Cancer Research Institute, San Francisco, CA 94143, USA;Laboratory of Physiological Chemistry, University of Groningen, 9712 KZ, Groningen, The Netherlands

关键词: Membrane fusion;    Glycolipid;    Lectin;    Calcium;    Liposome;   

DOI  :  10.1016/0014-5793(84)81021-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ca²⁺-induced fusion of phospholipid vesicles containing globoside (GL-4) or disialoganglioside (G_Dla) is several-fold slower than the fusion of the pure phospholipid vesicles. Lectins specific for these glycosphingolipids, soybean agglutinin and wheat germ agglutinin, respectively, enhance the rate of fusion when added to the vesicle suspension before the introduction of Ca²⁺. The enhancement depends on the lectin concentration and the time of preincubation with the lectin. We propose that lectins facilitate membrane fusion by inducing intermembrane contact, which is the first step in the overall process of membrane fusion, or by laterally phase separating the inhibitory glycolipids.

【 授权许可】

Unknown