The role of lipids and 17‐kDa protein in enhancing the recovery of O2 evolution in cholate‐treated thylakoid membranes

FEBS Letters

Toyoshima, Yoshinori¹  Imaoka, Akiko¹  Akabori, Kozo¹ 

[1] Faculty of Integrated Arts and Sciences, Hiroshima University, 1-1-89 Higashisenda-machi, Naka-ku, Hiroshima 730, Japan

关键词: Photosystem II;    O2 evolution;    Reconstitution;    Thylakoid lipid;    17-kDa protein Cl−;    Tricine;    N-tris(hydroxymethyl)methylglycine;    Mops;    3-(N-morpholino)propanesulfonic acid;    DPC;    1;    5-diphenylcarbazide;    DCIP;    2;    6-dichlorophenolindophenol;    PBQ;    phenyl-p-benzoquinone;    chl;    chlorophyll;    PS II;    Photosystem II;   

DOI  :  10.1016/0014-5793(84)81012-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The recovery of O₂ evolution of cholate-treated thylakoids induced by the simultaneous addition of the 17- and 23-kDa proteins was enhanced by the further addition of thylakoid total lipids up to about 75% of the non-depleted original broken thylakoids at the optimal concentration of the lipids, through reactivation of electron transfer between the sites of DPC donation and DCIP acceptance. Novel findings clarifing the cause of the discrepancies, with regard to the requirement for the 17-kDa protein on the O₂ evolution of thylakoid membranes, were also provided, i.e., when the assay of O₂ evolution for the reconstituted systems was carried out at a low level of NaCl (e.g., 0.1 mM), besides the 23-kDa protein, the 17-kDa protein was also required for O₂ evolution, however, at a higher level of NaCI (>5 mM) the 17-kDa protein was not. The results suggest that the 17-kDa protein takes the place of Cl⁻ or acts as a constituent protecting C⁻ in the Cl⁻ activating sites against fluctuation of the Cl⁻ level in the inner compartment of thylakoid membranes.

【 授权许可】

Unknown