Insertional inactivation of the psbO gene encoding the manganese stabilizing protein of photosystem II in the cyanobacterium Synechococcus PCC7942

FEBS Letters

Bockholt, Rainer¹  Masepohl, Bernd²  Pistorius, Elfriede K.¹ 

[1] Lehstuhl für Zellphysiologie, Fakultät für Biologie, Universität Bielefeld, 4800 Bielefeld 1, Germany;Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, 4800 Bielefeld 1, Germany

关键词: Photosystem II;    O2 evolution;    psbO gene;    Manganese stabilizing protein;    L-Amino acid oxidase;    L-AOX;    L-amino acid oxidase;    chl;    chlorophyll;    MSP;    manganese stabilizing protein;    PS;    photosystem;    WT;    wild-type;   

DOI  :  10.1016/0014-5793(91)81343-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Synechococcus PCC7942 mutant in which the psbO gene was inactivated by insertion of a chloramphenicol interposon and which did not contain any detectable manganese stabilizing protein in immunoblot experiments, was constructed. Such a Synechococcus mutant was able to grow under photoautotrophic conditions. Isolated thylakoid membranes from the mutant required addition of CaCl₂ and MnCl₂ for photosynthetic O₂ evolution, and the detectable L-amino acid oxidase activity in the isolated thylakoid membranes from the mutant was approximately four times higher than in wild-type thylakoids. The results are discussed with respect to our model suggesting that the water-oxidizing enzyme may have evolved from a flavoprotein with L-amino acid dehydrogenase/oxidase activity.

【 授权许可】

Unknown