Characterization of new enkephalin‐containing peptides in the adrenal medulla by immunoblotting

FEBS Letters

Liston, Dane¹  Rossier, Jean¹  Patey, Gilles¹ 

[1] Centre National de la Recherche Scientifique, Laboratoire de Physiologie Nerveuse, 91190 Gif-sur-Yvette, France

关键词: Enkephalin;    Synenkephalin;    Prohormone;    Adrenal medulla;    Immunoblotting;    Processing;   

DOI  :  10.1016/0014-5793(84)81146-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoblotting combined with radioimmunoassays (RIAs) directed specifically towards certain sequences of the proenkephalin molecule has been used to characterize the enkephalin-containing peptides (ECPs) present in the bovine adrenal medulla. Immunoblotting allowed the simultaneous visualization of all ECPs present in a crude extract of this gland. Combining this technique with RIAs we have been able to characterize a new high molecular mass ECP, a 23.3-kDa protein which contains the amino-terminal part of proenkephalin and ends with the sequence of Leu-enkephalin at its carboxy-terminus.

【 授权许可】

Unknown