The prohormone processing activity is enriched in a low‐density subpopulation of chromaffin granules

FEBS Letters

Fauchère, Jean-Luc¹  Maret, Gérard E.¹ 

[1] Institute of Biotechnology, Swiss Federal Institute of Technology (ETH), CH8093 Zurich, Switzerland

关键词: Chromaffin granule Prohormone processing;   

DOI  :  10.1016/0014-5793(86)80171-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine adrenomedullary chromaffin granules can be separated into two subpopulations by differential centrifugation. The subpopulation which sediments at the interface of two sucrose layers, 1.6 and 1.8 M respectively, is found to be enriched about 10-times in prohormone processing activity, as measured by in vitro degradation of synthetic peptide substrates. The enhanced proteolytic activity is not due to lysosomal contaminations which are very low and only slightly increased in the more active fraction. The low density of the enriched subpopulation suggests that we are dealing with immature granules. The physiological implications of this finding are discussed. Furthermore, the enriched fraction can be used as the starting material for the isolation ofproenkephalin processing enzymes.

【 授权许可】

Unknown