Intracellular pH measurements using the fluorescence of 9‐aminoacridine

FEBS Letters

Lazdunski, Michel¹  Frelin, Christian¹  Vigne, Paul¹ 

[1]Centre de Biochimie, Centre National de la Recherche Scientifique, Faculté des Sciences, Parc Valrose, 06034 Nice Cédex, France

关键词: Intracellular pH;    9-Aminoacridine;    Nigericin;    Monensin;    Skeletal muscle;    9-AA;    9-aminoacridine;    DMO;    dimethyloxazolidinedione;    pHi;    internal pH;   

DOI  :  10.1016/0014-5793(84)81140-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

9-Aminoacridine is a fluorescent weak base which distributes across the plasma membrane according to the pH gradient, and can be used to monitor changes in intracellular pH in eukaryotic cells. A calibration technique using the ionophore nigericin is described which allows us to measure intracellular pH values of chick skeletal muscle cells.

【 授权许可】

Unknown