Role of intestinal brush border membrane aminopeptidase N in dipeptide transport

FEBS Letters

Antonov, V.K.¹  Zilberman, M.I.¹  Bessmertnaya, L.Ya.¹  Mikhailova, A.G.¹  Vorotyntseva, T.I.¹ 

[1] Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, 117988 Moscow, USSR

关键词: Brush border membrane;    Aminopeptidase N;    Dipeptide;    Amino acid transport;   

DOI  :  10.1016/0014-5793(84)80493-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinetics of uptake of radioactive label from [U-¹⁴C]Gly, L-[4,5-³H]Leu and the dipeptide [¹⁴C]Gly—L-[4,5-³H]Leu by the brush border membrane vesicles of porcine small intestine have been studied. The effect of aminopeptidase N inhibitors and leucine-binding protein on accumulation rates has also been tested. Comparison of the kinetic parameters for uptake and hydrolysis of Gly—L-Leu makes it possible to conclude that the dipeptide transfer includes two conjugated steps, viz., hydrolysis catalysed by aminopeptidase N and transport of the resultant free amino acids by a specific carrier.

【 授权许可】

Unknown