Primary structure of the major glycans of the N‐acetyllactosamine type derived from the human immunoglobulins M from two patients with Waldenström's macroglobulinemia

FEBS Letters

Vliegenthart, J.F.G.¹  Cahour, A.³  Debeire, P.²  Van Halbeek, H.¹  Hartmann, L.³  Montreuil, J.² 

[1] Department of Bio-Organic Chemistry, University of Utrecht, Croesestraat 79, NL-3522 AD Utrecht, The Netherlands;Université des Sciences et Techniques de Lille I, Laboratoire de Chimie Biologique (LA CNRS 217), F-59655 Villeneuve d'Ascq Cédex, France;Université Pierre et Marie Curie, Laboratoire de Chimie Clinique et Biologie Moléculaire (U. 202 INSERM and ERA CNRS 696), 15, rue de l'Ecole de Médicine, F-75270 Paris Cédex 06, France

关键词: Immunoglobulin M;    Waldenström's macroglobulinemia;    N-Glycosidic glycan;    500 MHz 1H-NMR spectroscopy;    Fuc;    L-fucose;    Gal;    D-galactose;    Man;    D-mannose;    GlcNAc;    N-acetyl-D-glucosamine;    NeuAc;    N-acetylneuraminic acid;    IgM;    immunoglobulin M;    Con A;    concanavalin A;   

DOI  :  10.1016/0014-5793(84)81341-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary structure of the major compounds was elucidated on the basis of carbohydrate analysis, methylation analysis, including mass-spectrometry, and 500 MHz ¹H-NMR spectroscopy. For both patients, this appeared to be a monosialyl monofucosyl biantennary structure; the compounds differed by the presence of an intersecting N-acetylglucosamine residue.

【 授权许可】

Unknown