FEBS Letters | |
Primary structure of the major glycans of the N‐acetyllactosamine type derived from the human immunoglobulins M from two patients with Waldenström's macroglobulinemia | |
Vliegenthart, J.F.G.1  Cahour, A.3  Debeire, P.2  Van Halbeek, H.1  Hartmann, L.3  Montreuil, J.2  | |
[1] Department of Bio-Organic Chemistry, University of Utrecht, Croesestraat 79, NL-3522 AD Utrecht, The Netherlands;Université des Sciences et Techniques de Lille I, Laboratoire de Chimie Biologique (LA CNRS 217), F-59655 Villeneuve d'Ascq Cédex, France;Université Pierre et Marie Curie, Laboratoire de Chimie Clinique et Biologie Moléculaire (U. 202 INSERM and ERA CNRS 696), 15, rue de l'Ecole de Médicine, F-75270 Paris Cédex 06, France | |
关键词: Immunoglobulin M; Waldenström's macroglobulinemia; N-Glycosidic glycan; 500 MHz 1H-NMR spectroscopy; Fuc; L-fucose; Gal; D-galactose; Man; D-mannose; GlcNAc; N-acetyl-D-glucosamine; NeuAc; N-acetylneuraminic acid; IgM; immunoglobulin M; Con A; concanavalin A; | |
DOI : 10.1016/0014-5793(84)81341-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary structure of the major compounds was elucidated on the basis of carbohydrate analysis, methylation analysis, including mass-spectrometry, and 500 MHz 1H-NMR spectroscopy. For both patients, this appeared to be a monosialyl monofucosyl biantennary structure; the compounds differed by the presence of an intersecting N-acetylglucosamine residue.
【 授权许可】
Unknown
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