Precursor of the myo‐inositol‐binding protein of a Pseudomonas species

FEBS Letters

Feiss, Dominique¹  Deshusses, Jacques¹  Belet, Monique¹ 

[1] Département de Biochimie, Université de Genève, 30 quai E. Ansermet, 1211 Genève 4, Switzerland

关键词: Pseudomonas;    Precursor protein;    Periplasmic protein myo-Inositol;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phosphate saline buffer (0.05 M sodium phosphate;    0.15 M NaCl;    pH 7.4);    TAME;    p-tosyl-L-arginine methyl ester;   

DOI  :  10.1016/0014-5793(84)81392-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biosynthesis of the periplasmic binding protein of the myo-inositol high affinity transport system in a Pseudomonas strain involves the synthesis of a precursor of 2 kDa heavier than the mature form (30 kDa). The identification of the precursor is based on the comparison of the fingerprints of the two proteins and on kinetics in pulse-chase experiments.

【 授权许可】

Unknown