FEBS Letters | |
Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor | |
Chow, Virginia1  Dixon, James D.2  Murray, Robert K.1  Kurosky, Alexander2  | |
[1] Departments of Biochemistry and Pathology and the Institute of Medical Science, University of Toronto, Toronto, Ontario M5S 1A8, Canada;Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX 77550, USA | |
关键词: Haptoglobin; Cell-free translation; Signal sequence; Precursor protein; Hp; haptoglobin; PAGE; polyacrylamide gel electrophoresis; Pth; phenylthiohydantoin; HPLC; high performance liquid chromatography; SDS; sodium dodecyl sulfate; | |
DOI : 10.1016/0014-5793(83)80623-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of M r 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.
【 授权许可】
Unknown
【 预 览 】
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RO201912020284060ZK.pdf | 570KB | download |