期刊论文详细信息
FEBS Letters
Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor
Chow, Virginia1  Dixon, James D.2  Murray, Robert K.1  Kurosky, Alexander2 
[1] Departments of Biochemistry and Pathology and the Institute of Medical Science, University of Toronto, Toronto, Ontario M5S 1A8, Canada;Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX 77550, USA
关键词: Haptoglobin;    Cell-free translation;    Signal sequence;    Precursor protein;    Hp;    haptoglobin;    PAGE;    polyacrylamide gel electrophoresis;    Pth;    phenylthiohydantoin;    HPLC;    high performance liquid chromatography;    SDS;    sodium dodecyl sulfate;   
DOI  :  10.1016/0014-5793(83)80623-1
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of M r 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.

【 授权许可】

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