Two‐dimensional gel electrophoresis of proteins released from sea urchin nuclei by limited digestion with DNase I

FEBS Letters

Takahashi, Kazuhide¹ 

[1] Department of Biology, Faculty of Science, Tokyo Metropolitan University, Setagaya, Tokyo 158, Japan

关键词: DNase I digestion;    Nuclear protein;    Sea urchin;   

DOI  :  10.1016/0014-5793(84)80311-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The morula and the mesenchyme blastula nuclei contained approx. 30 nuclear proteins which were preferentially released by limited digestion with DNase I, but no proteins were released from sperm nuclei. While most of the proteins released by DNase I digestion were common to the two embryonic stages, 2 and 6 proteins were specific or enriched in morulae and mesenchyme blastulae, respectively.

【 授权许可】

Unknown