Effects of FSH on acidic nuclear protein synthesis in cultured pig Sertoli cells

FEBS Letters

Dazord, A.¹  Perrard, M.H.¹  Saez, J.M.¹ 

[1]INSERM U.162, Hôpital Debrousse, 29 Rue Soeur Bouvier, 69322 Lyon Cédex 05, France

关键词: Sertoli cell;    FSH;    Nuclear protein;    Primary culture;   

DOI  :  10.1016/0014-5793(84)80204-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the effect of FHS on nuclear protein synthesis by a primary culture of immature porcine Sertoli cells. The cells were cultured in a chemically defined medium and treated with FSH (50 ng/ml) for various periods (4, 8, 25 and 64 h). About 65 spots (pH_i < 8) were identified by two-dimensional polyacrylamide gel electrophoresis of the radiolabelled nuclear proteins. After 25 h of FHS treatment, we observed an increase of 5 proteins, a slight decrease of two and a disappearance of one. For the short periods of FSH treatment (4 and 8 h), no effect of FHS on nuclear protein synthesis was observed. After 64 h of FSH treatment, the synthesis of all nuclear proteins appears to be decreased.

【 授权许可】

Unknown