Inactivation of beef heart mitochondrial F1‐ATPase by the 2′,3′‐dialdehyde derivatives of adenine nucleotides

FEBS Letters

Satre, Michel¹  Fernandes de Melo, Dirce¹  Vignais, Pierre V.¹ 

[1] Laboratoire de Biochimie (CNRS/ERA 903 et INSERM U.191), Département de Recherche Fondamentale, Centre d'Etudes Nucléaires 85X, 38041 Grenoble Cédex, France

关键词: F1-ATPase;    Dialnucleotide;    Affinity labeling;    Enzyme inactivation;    F1;    beef heart soluble mitochondrial ATPase;    Tra;    triethanolamine;    oAdN;    2′;    3′-dialdehyde derivatives of ATP;    ADP and AMP (oATP;    oADP;    oAMP);   

DOI  :  10.1016/0014-5793(84)80297-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Beef heart mitochondrial F₁-ATPase was inactivated by the 2′,3′-dialdehyde derivatives of ATP, ADP and AMP (oATP, oADP, oAMP). In the absence of Mg²⁺, inactivation resulted from the binding of 1 mol nucleotide analog per active unit of F₁. The most efficient analog was oADP, followed by oAMP and oATP. Complete inactivation was correlated with the binding of about 11 mol [¹⁴C]oADP/mol F₁. After correction for non-specific labeling, the number of specifically bound [¹⁴C]oADP was 2–3 mol per mol F₁. By SDS-polyacrylamide gel electrophoresis, [¹⁴C]oADP was found to bind covalently mainly to the α and β subunits. In the presence of Mg²⁺, oATP behaved as a substrate and was slowly hydrolyzed.

【 授权许可】

Unknown