Reconstitution of 50 S ribosomal subunits from Bacillus stearothermophilus with 5 S RNA from spinach chloroplasts and low‐M r RNA from mitochondria of Locusta migratoria and bovine liver

FEBS Letters

Subramanian, Alap R.⁴  Vogel, Detlef W.²  Pieler, Tomas²  Erdmann, Volker A.²  Kleinow, Walter¹  O'Brien, Thomas W.³  Bartsch, Marius⁴  Hartmann, Roland K.² 

[1] II. Lehrstuhl: Tierphysiologie, Weyertal 119, D-5000 Köln 41, FRG;Institut für Biochemie, Freie Universität Berlin, Thielallee 69-73, D-1000 Berlin 33, Germany;Department of Biochemistry and Molecular Biology, Box J-245, University of Florida, Gainesville, FL 32610, USA;Max-Planck-Institut für Molekulare Genetik, Ihnestraße 63-73, D-1000 Berlin 33, Germany

关键词: Chloroplast 5 S rRNA;    Mitochondrial 5 S rRNA;    50 S ribosome;    Reconstitution;    Protein synthesis;    Evolution;   

DOI  :  10.1016/0014-5793(84)80291-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Reconstitution experiments with 50 S ribosomal subunits from Bacillus stearothermophilus demonstrate that spinach chloroplast 5 S rRNA can be incorporated into the bacterial ribosome and yield biologically active particles, thereby establishing the eubacterial nature of chloroplast 5 S rRNA. In contrast, mitochondria from Locusta migratoria or bovine liver do not appear to contain discrete, low-M _r, RNAs, which can replace 5 S rRNA in the functional reconstitution of B. stearothermophillus ribosomes.

【 授权许可】

Unknown