Proinsulin‐like material in mouse foetal brain cell cultures

FEBS Letters

Renwick, Alistair G.C.¹  Birch, Nigel P.¹  Christie, David L.¹ 

[1]Department of Biochemistry, University of Auckland, Private Bag, Auckland, New Zealand

关键词: Proinsulin;    Insulin;    Cultured brain cell;    HPLC;   

DOI  :  10.1016/0014-5793(84)80266-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two main forms of immunoreactive insulin have been identified in cultures of foetal mouse brain using HPLC and gel filtration. The major component which resembled proinsulin was converted by trypsin to the minor form which was similar to authentic pancreatic insulin in chromatographic behaviour. Both components showed immunological properties comparable to insulin and proinsulin including sensitivity of the former to reduction and alkylation.

【 授权许可】

Unknown