ATP and other purine nucleotides stimulate the inactivation of ornithine transcarbamylase by broken lysosomes

FEBS Letters

Navarro, Alfonso¹  Grisolía, Santiago¹ 

[1] Istituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Amadeo de Saboya 4, Valencia 10, Spain

关键词: Ornithine transcarbamylase;    Lysosome;    ATP;    Carbamoyl phosphate;    OTC;    ornithine transcarbamylase;    β-NAGASE;    β-N-acetyl-D-glucosaminidase;    BSA;    bovine serum albumin;    ϵ-ATP;    1-N 6-ethenoadenosine 5′-triphosphate;    MSH;    β-mercaptoethanol;   

DOI  :  10.1016/0014-5793(84)80138-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ornithine transcarbamylase (OTC) is very stable in rat liver homogenates, or mitochondria. However, pure OTC from rat or beef liver is inactivated by broken lysosomes. ATP and a number of purine nucleotides, particularly ϵ-ATP, stimulate the inactivation of OTC by broken lysosomes. Inactivation of OTC by trypsin and elastase is also stimulated by ϵ-ATP. Carbamoyl phosphate protects OTC against proteolytic attack by elastase or by broken lysosomes. Thus, environmental factors, e.g., ATP and carbamoyl phosphate, appear to influence OTC stability to proteases.

【 授权许可】

Unknown