Organ‐specific properties of cytochromes P‐450s21 (steroid 21‐hydroxylases) of liver and adrenocortical microsomes

FEBS Letters

Ichikawa, Yoshiyuki¹  Tsubaki, Motonari¹  Hiwatashi, Atsuo¹ 

[1] Department of Biochemistry, Kagawa Medical School, Kita-gun, Kagawa 761-07, Japan

关键词: Cytochrome P-450;    Steroid 21-hydroxylase;    Microsomal monooxygenase;    Microsomal mixed function oxidase;    Cytochrome P-450s21;    SDS;    sodium dodecyl sulfate;    cytochrome P-450s21;    cytochrome P-450 catalyzing steroid 21-hydroxylation;   

DOI  :  10.1016/0014-5793(84)80847-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Organ specificities of cytochrome P-450_s21 (steroid 21-hydroxylase) were investigated. The substrate specificity of the liver cytochrome P-450_s21 was found to be different from that of adrenocortical cytochrome P-450_s21. The steroid 21-hydroxylase activity of liver microsomes decreased with treatment by sodium phenobarbital or β-naphthoflavone and was inhibited by anti-cytochrome b ₅ immunoglobulin, although that of adrenocortical microsomes was not. The liver cytochrome P-450_s21 was immunochemically similar to adrenocortial cytochrome P-450_s21. The bovine liver and adrenocortical cytochromes P-450_s21 showed immunoprecipitin lines against antibody to bovine adrenocortical cytochrome P-450_s21. The molecular masses of the bovine liver and adrenocortical cytochromes P-450_s21 were 60 ± 1 and 50 ± 1 kDa, respectively. The content of cytochrome P-450_s21 and its percentage for the total microsomal cytochrome P-450 were immunochemically determined using the antibody to the bovine adrenocortical cytochrome P-450_s21.

【 授权许可】

Unknown