Solid‐phase biosynthesis on high performance thin‐layer plates of blood group glycosphingolipids II

FEBS Letters

Samuelsson, Bo E.¹ 

[1] Department of Medical Biochemistry, University of Göteborg, PO Box 33031, S-40033 Göteborg Sweden

关键词: Glycolipid;    Fucolipid;    Blood group;    Thin-layer chromatography;    Glycosyltransferase;    Solid-phase biosynthesis;   

DOI  :  10.1016/0014-5793(84)80830-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new method for the study of carbohydrate chain biosynthesis of amphipathic glycosphingolipids is presented. The method takes advantage of the thin-layer plate which is used as a solid matrix of precursor glycosphingolipids in biosynthetic experiments after chromatographic development of the precursor sample. The method is shown to be simple, fast and sensitive. It minimizes the risk of adding unwanted exogenous precursors and abolishes the need for tedious purification of products after incubation. The method opens up new possibilities for the biosynthetic study of mixtures of glycosphingolipids.

【 授权许可】

Unknown