Reconstitution of solubilised brain D2 dopamine receptors into phospholipid vesicles

FEBS Letters

Wheatley, M.¹  Strange, P.G.¹ 

[1] Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH, England

关键词: D2 dopamine receptor;    Solubilisation;    Reconstitution [3H]Spiperone binding;   

DOI  :  10.1016/0014-5793(84)80119-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

D₂ dopamine receptors have been solubilised from bovine caudate nucleus using cholate/sodium chloride in the presence of soyabean phospholipid. Reconstitution of the receptors into soyabean phospholipid vesicles has been achieved by dialysis to remove detergent and salt. The receptors are truly reconstituted as judged by sedimentation, electron microscopy, heat stability and analysis on sucrose density gradients. The ligand-binding properties of the reconstituted receptors resemble those of the solubilised preparation.

【 授权许可】

Unknown