Vacuoles are not the sole compartments of proteolytic enzymes in yeast

FEBS Letters

Wolf, Dieter H.¹  Emter, Othniel¹ 

[1] Biochemisches Institut, Universität Freiburg, Hermann-Herder-Str. 7, D-7800 Freiburg i.Br., FRG

关键词: Yeast;    Mutant;    Compartmentation;    Vacuole;    Endopeptidase;    Exopeptidase;    Nan;    4-nitroanilide;    Ac;    acetyl;    Bz;    benzoyl;    Cbz;    benzyloxycarbonyl;    MeO;    methoxy;    Suc;    succinyl;    Tos;    tosyl;    Mes;    2-(N-morpholino)ethanesulfonic acid;    n.d.;    not determined;    unless otherwise stated amino acids were of L-configuration. Genetic markers: pra1;    proteinase A deficiency;    prb1;    proteinase B deficiency;    prc1;    carboxypeptidase Y deficiency;    cps1;    carboxypeptidase S deficiency;    ade;    adenine auxotrophy;   

DOI  :  10.1016/0014-5793(84)80104-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Localization in vacuoles, the lysosome-like organelle of yeast, was checked for several newly detected proteolytic enzymes. While aminopeptidase Co and carboxypeptidase S were found in vacuoles, proteinase D and proteinase E as well as a variety of other proteolytic activities detectable with the aid of chromogenic peptide substrates do not reside in this cell compartment.

【 授权许可】

Unknown