Effects of growth hormone‐releasing factor, somatostatin and dopamine on growth hormone and prolactin secretion from cultured ovine pituitary cells

FEBS Letters

Law, Greg J.¹  Ray, Keith P.¹  Wallis, Michael¹ 

[1] Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, England

关键词: Growth hormone releasing factor;    Growth hormone;    Prolactin;    Somatostatin;    Dopamine;    Cyclic AMP;    GRF;    growth hormone releasing factor;    SRIF;    somatostatin;    DA;    dopamine;    GH;    growth hormone;    VIP;    vasoactive intestinal peptide;    Hepes;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(84)80070-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH₂) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 μM) completely blocked the maximal GRF (1 nM)-stimulated secretion without a significant effect on cyclic AMP levels. Dopamine (0.1 μM) inhibited the GRF-elevated GH secretion by 50% and lowered cyclic AMP levels by 30%. Dopamine (0.1 μM) inhibition of basal prolactin secretion was not affected by GRF (1 nM). The data support the hypothesis that cyclic AMP is involved in the action of GRF but suggest that somatostatin can inhibit GRF-induced secretion of GH independently of cyclic AMP.

【 授权许可】

Unknown