Photoaffinity labeling of acetylcholine receptor in millisecond time scale

FEBS Letters

Hucho, Ferdinand¹  Muhn, Peter¹  Fahr, Alfred¹ 

[1] Institut für Biochemie und Institut für Atom- und Festkörperphysik, Freie Universität Berlin, Fabeckstr. 34, 1000 Berlin 33, FRG

关键词: Acetylcholine receptor;    Photoaffinity labeling;    Ion channel;    TPMP+;   

DOI  :  10.1016/0014-5793(84)80061-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photoaffinity labeling of acetylcholine receptors can be performed with a time resolution allowing to discriminate reaction sites within the receptor protein in its different functional states. This is achieved by a combination of a stopped-flow apparatus with a high energy pulse laser. The photoaffinity label used is the lipophilic cation [³H]TPMP⁺ which has been shown to be a non-competitive antagonist and a specific ion channel blocker. AChR in its resting (channel closed) and active (channel open) state incorporates the label mainly into the α-polypeptide chain of the receptor. Only several hundred milliseconds after mixing AChR with agonist labeling of δ-chains becomes significant.

【 授权许可】

Unknown