FEBS Letters | |
Preparation of an ϵ‐deficient chloroplast coupling factor 1 having a high ATPase activity | |
Rubinstein, Menachem1  Finel, Moshe2  Pick, Uri2  | |
[1] Virology Department, The Weizman Institute of Science, Rehovot 76100, Israel;Biochemistry Department, The Weizman Institute of Science, Rehovot 76100, Israel | |
关键词: Chloroplast coupling factor 1 (CF1); ATPase regulation; ϵ-Subunit; High-performance liquid chromatography; CF1; chloroplast coupling factor 1; DTT; dithiothreitol; HPLC; high-performance liquid chromatography; OG; octyl-D-glucopyranoside (octylglucoside); SDS; sodium dodecyl sulfate; PAGE; polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(84)80050-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A modification of the chloroform extraction preparation of CF1, by elevating the pH during the DEAE-Sephadex purification step to 7.8 yields a 5-subunit CF1. Different 4-subunit CF1 preparations deficient in either δ- or ϵ-subunits, as well as pure β-subunits are obtained by fractionation of intact CF1 complex by anion-exchange high-performance liquid chromatography. Unlike the 5-subunit latent enzyme, ϵ-deficient CF1 has high Ca-ATPase activity which can be inhibited by purified ϵ-subunits. After trypsin or octylglucoside activations all preparations have identical high ATPase activity. These results suggest that the subunit regulates CF1-ATPase activity.
【 授权许可】
Unknown
【 预 览 】
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RO201912020285112ZK.pdf | 434KB | download |