Protein synthesis by isolated chloroplasts of Chlamydomonas reinhardii

FEBS Letters

Boschetti, Arminio¹  Leu, Stefan¹  Mendiola-Morgenthaler, Leticia¹ 

[1] Institut für Biochemie, Universität Bern, Freiestrasse 3, CH-3012 Bern, Switzerland

关键词: Chlamydomonas reinhardii;    Isolated chloroplast;    Chloroplast protein synthesis;   

DOI  :  10.1016/0014-5793(84)80037-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chloroplasts which have been isolated from the cell wall mutant cw15-2 of Chlamydomonas reinhardii are active in light-dependent, in organello protein synthesis. Certain characteristics of protein synthesis in this system were tested, such as time course of amino acid incorporation, sensitivities to Mg²⁺ as well as to specific inhibitors, and found to be typical of chloroplasts isolated from higher plants. The major products made were identified as the large subunit of ribulose-1,5-biphosphate carboxylase and the 32-kDa rapidly labelled membrane protein.

【 授权许可】

Unknown