A chloroplast system capable of translating heterologous mRNAs

FEBS Letters

Camerino, Giovanna¹  Ciferri, Orio¹  Savi, Antonella¹ 

[1] Institute of Microbiology and Plant Physiology, University of Pavia, Via S. Epifanio, 14, 27100 Pavia, Italy

关键词: Chloroplast protein synthesis;    Phage MS-2 RNA translation;    Translation in vitro;   

DOI  :  10.1016/0014-5793(82)81311-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A system capable of incorporating amino acids into protein has been prepared from chloroplasts isolated from spinach leaves. The activity of the system is inhibited by chloramphenicol or RNase but not by rifampycin or cycloheximide. After reducing the endogenous activity by treatment with micrococcal nuclease, the system responds to homologous or heterologous mRNAs. The RNA from MS-2 phage is translated faithfully as demonstrated by the isolation from the translation products of a protein with the same mobility of the phage coat protein. Partial proteolytic digestion confirmed that the protein synthesized in vitro is indeed the phage protein.

【 授权许可】

Unknown