A putative primer for the replication of cauliflower mosaic virus by reverse transcription is virion‐associated

FEBS Letters

Turner, David S.¹  Covey, Simon N.¹ 

[1] Department of Virus Research, John Innes Institute, Colney Lane, Norwich, Norfolk NR4 7UH England

关键词: DNA plant virus;    CaMV;    tRNAmet;    Reverse transcription;   

DOI  :  10.1016/0014-5793(84)80187-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated, from a cauliflower mosaic virus (CaMV) preparation, an RNA species (75 nucleotides) covalently linked to the 5′-end of the CaMV DNA fragment (sa-DNA) which is complementary to the CaMV 35 S RNA transcript 5′-end. Site-specific cleavage located a unique m⁷G residue in the RNA moiety of sa-DNA suggesting that it is host cell tRNA^met _i sa-DNA is reminiscent of retrovirus ‘strong-stop“ DNA and is evidence fo the reverse transcription model of CaMV replication.

【 授权许可】

Unknown