Mobilization of arachidonic acid between diacyl and ether phospholipids in rabbit alveolar macrophages

FEBS Letters

Waku, Keizo¹  Sugiura, Takayuki¹  Fukui, Junko¹  Katayama, Osamu¹  Nakagawa, Yasuhito¹ 

[1]Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-01, Japan

关键词: Macrophage;    Arachidonic acid;    Ether phospholipid;    Alkylacyl derivative;    Alkenylacyl derivative;    20:4;    arachidonic acid;    18:2;    linoleic acid;    CGP;    choline glycerophospholipids;    EGP;    ethanolamine glycerophospholipids;    IGP;    inositol glycerophospholipids;    GPC;    glycerophosphocholine;    GPE;    glycerophosphoethanolamine;    BSA;    bovine serum albumin;    Hepes;    N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid;    PAF;    platelet-activing factor;   

DOI  :  10.1016/0014-5793(84)80184-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [³H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC). After the removal of free 20:4, the radioactivity was gradually lost from diacyl GPC. Concomitantly, the radioactivities in alkylacyl GPC and alkenylacyl glycerophosphoethanolamine (GPE) were increased, indicating that 20:4 was mobilized from diacyl GPC to alkylacyl GPC and alkenylacyl GPE. The mobilization was considered to be a 20:4-specific event. The gradual accumulation of 20:4 in ether phospholipids leads to a high abundance of 20:4 in these lipids. These results suggest metabolic relationships between 20:4 and ether phospholipids, including platelet-activating factor (PAF).

【 授权许可】

Unknown