Fluorescence polarization study of tertiary structure of DNA within bacteriophage γ

FEBS Letters

Shurdov, M.A.¹  Gruzdev, A.D.¹ 

[1] All-Union Research Institute of Molecular Biology, Kol'tsovo Settlement, Novosibirsk Region, 633159 Novosibirsk District, USSR

关键词: DNA packing;    Phage γ;    Acridine orange;    Fluorescence polarization;    Bacteriophage γ;   

DOI  :  10.1016/0014-5793(84)80176-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fluorescence polarization of acridine orange-stained oriented γ phages was measured. The parameters of DNA packing within the phage head and were calculated (φ, angle between the direction of a small segment of DNA and the phage axis). It is shown that simple models of γ phage DNA tertiary structure are not consistent with calculated values. A new model is proposed.

【 授权许可】

Unknown