Purification of three rat atrial natriuretic factors and their amino acid composition

FEBS Letters

Garcia, R.¹  Lazure, C.¹  Cantin, M.¹  Chrétien, M.¹  Thibault, G.¹  Genest, J.¹  Seidah, N.G.¹ 

[1] Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada

关键词: Atrial natriuretic factor;    Natriuresis;    Diuresis;    Peptide purification;   

DOI  :  10.1016/0014-5793(83)80303-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A natriuretic factor has been described in the specific granules of rat atria. We have purified three factors which seem to be low-M _r peptides. They have been purified by means of acid extraction, octadecyl Sep-Pak cartridges, and chromatography on Bio-Gel P-10, CM Bio-Gel A, Mono S and reverse-phase high-performance liquid chromatography columns. The factors contain 26, 31 and 33 amino acids and may have been partially degraded during isolation. They are all 3 biologically active and the shorter one is the most active with a specific activity of 450000 units/mg.

【 授权许可】

Unknown