Chick retinal pigment epithelium

FEBS Letters

Koh, Shay-Whey M.¹  Chader, Gerald J.¹ 

[1] National Eye Institute, National Institutes of Health, Bethesda, MD 20205, USA

关键词: Histamine;    Cyclic AMP;    Cimetidine;    Diphenhydramine;    Pyrilamine;    Retinal pigment epithelium;   

DOI  :  10.1016/0014-5793(83)80301-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Histamine elevates the intracellular cyclic AMP levels in cultured embryonic chick retinal pigment epithelium. The half-maximal activity is 6 × 10⁻⁶ M. The effect of histamine is mediated by H₂ receptors, i.e., inhibited by the H₂ antagonist cimetidine and not affected by the H₁ antagonists diphenhydramine and pyrilamine. The inhibition constant (K ₁) of cimetidine is 1.3 × 10⁻⁸ M. Thus, the present system offers the opportunity of studying the nature of coupling between histamine receptors and adenylate cyclase under controlled conditions in a homogeneous cell type.

【 授权许可】

Unknown