Histamine interaction on surface recognition sites of H2‐type in parietal and non‐parietal cells isolated from the guinea pig stomach

FEBS Letters

Rosselin, Gabriel¹  Bouhours, Danièle¹  Gespach, Christian²  Bouhours, Jean-François¹ 

[1] Université Claude Bernard Lyon 1, Laboratoire de Biochimie des Membranes, 69261 Lyon Cedex, France;Unité de Recherches de Diabétologie et d'Etudes Radioimmunologiques des Hormones Protéiques, U.55 (INSERM), Hôpital Saint-Antoine, 184, rue du Faubourg Saint-Antoine, 75571 Paris Cedex 12 France

关键词: Histamine;    Fundic;    antral cell;    H2 receptor;    Cyclic AMP;    Gastric secretion;   

DOI  :  10.1016/0014-5793(82)81077-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In gastric cells isolated by pronase digestion from the guinea pig, histamine stimulated cAMP production in 3 fundic cell fractions (EC ₅₀ = 1.6–2 × 10⁻⁴ M) enriched in parietal (94%), peptic (63%) and mucous cells (87%) as well as in antral cells (EC ₅₀ = 4 × 10⁻⁴ M) that are devoid of parietal cells. Histamine stimulations were completely inhibited by the H₂ antagonist cimetidine (K _i = 0.27–0.57 × 10⁻⁶ M) or by the H₁ antagonist diphenhydramine, but at 100-times lower potency (K _i = 22–45.7 × 10⁻⁶ M), indicating the presence of histamine H₂ receptors in parietal and nonparietal cells of the guinea pig gastric mucosa.

【 授权许可】

Unknown